Pitie-Salpetriere University Hospital 1 article published in JoVE Neuroscience Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments Elisa Mazuir1, Anne-Laure Dubessy1,2, Loane Wallon1,3, Marie-Stéphane Aigrot1, Catherine Lubetzki1,2, Nathalie Sol-Foulon1 1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.
