Centre Hospitalier Universitaire (CHU) de Reims 1 article published in JoVE Biology An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures Charlie Colin-Pierre1,2,3, Oussama El Baraka3, Laurent Ramont1,2,4, Stéphane Brézillon1,2 1Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 2UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, Université de Reims Champagne-Ardenne Reims, 3BASF Beauty Care Solutions, 4Service Biochimie-Pharmacologie-Toxicologie, Centre Hospitalier Universitaire (CHU) de Reims In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.
