Universite Cote d’Azur, CHU de Nice 1 article published in JoVE Biology Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer Christophe Bontoux1,2,3,4,5, Virginie Lespinet-Fabre1,2,3,4, Olivier Bordone1,2,3,4, Virginie Tanga1,2,3,4, Maryline Allegra1,2,3,4, Myriam Salah1,2,3,4, Salomé Lalvée1,2,3,4, Samantha Goffinet1,2,3,4, Jonathan Benzaquen3,4,5,6, Charles-Hugo Marquette3,4,5,6, Marius Ilié1,2,3,4,5, Véronique Hofman1,2,3,4,5, Paul Hofman1,2,3,4,5 1Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d’Azur, CHU de Nice, 2Institut Hospitalo-Universitaire (IHU), RespirERA, Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, 4Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne, Université Côte d'Azur, 6 The increase of molecular biomarkers to be tested for non-squamous non-small cell lung cancer (NS-NSCLC) care management has prompted the development of fast and reliable molecular detection methods. We describe a workflow for genomic alteration assessment for NS-NSCLC patients using an ultra-fast-next generation sequencing (NGS) approach.
