Francis Crick Institute 6 articles published in JoVE Immunology and Infection Assessing Respiratory Immune Responses to Haemophilus Influenzae Lovisa Dousha1,2, Roleen Sharma1,2, Steven Lim2,3, James Ngui2,4, Ashley M. Buckle5, Paul T. King1,2 1Monash Lung and Sleep, Monash Medical Centre, 2Monash University Department of Medicine, Monash Medical Centre, 3Flow Cytometry Science Technology Platform, Francis Crick Institute, 4Clinical Immunology, Monash Medical Centre, 5Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University Haemophilus influenzae induces inflammation in the respiratory tract. This article will focus on the use of flow cytometry and confocal microscopy to define immune responses by phagocytes and lymphocytes in response to this bacterium. Environment Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica Aki Masunaga1, Andrew W. Liu1, Yongkai Tan1, Andrew Scott1, Nicholas M. Luscombe1,2,3 1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment, University College London Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions. Immunology and Infection Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment Rebeca Jimeno1,2, Phillip M. Brailey1,2, Patricia Barral1,2 1 Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR. Biology Environmental Screening of Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa in Zebrafish Systems Jean-Philippe Mocho1, Darren J. Martin1, Mollie E. Millington1, Yolanda Saavedra Torres1 1Biological Research Facility, The Francis Crick Institute This protocol describes the use of sump swabs and sludge analysis of zebrafish systems, which leads to increased detection compared to the sole use of sentinels to detect pathogens such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa. A system to monitor P. tomentosa eggs in quarantine is also proposed. Developmental Biology Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging Diana Passaro*1, Ander Abarrategi*1, Katie Foster1, Linda Ariza-McNaughton1, Dominique Bonnet1 1Haematopoietic Stem Cell Laboratory, The Francis Crick Institute A method to create and live-image different humanized bone-marrow niches in mice is presented. Based on the supportive niche created by human mesenchymal cells, the addition of human endothelial cells induces the formation of human vessels, while the addition of rhBMP-2 induces the formation of human-mouse chimeric mature bone tissue. Neuroscience In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ Tobias Ackels1,2, Daniela R. Drose1, Marc Spehr1 1Department of Chemosensation, Institute for Biology II, RWTH Aachen University, 2Mill Hill Laboratory, The Francis Crick Institute Here, we describe a physiological approach that allows identification and in-depth analysis of a defined population of sensory neurons in acute coronal tissue slices of the mouse vomeronasal organ using whole-cell patch-clamp recordings.
