University of Southampton 8 articles published in JoVE Biochemistry Fixed Target Serial Data Collection at Diamond Light Source Sam Horrell1, Danny Axford1, Nicholas E. Devenish1, Ali Ebrahim1, Michael A. Hough2, Darren A. Sherrell1,3, Selina L. S. Storm1,4, Ivo Tews5, Jonathan A. R. Worrall2, Robin L. Owen1 1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences, University of Essex, 3X-ray Science Division, Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences, University of Southampton We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24. Chemistry A New Straightforward Method for Lipophilicity (logP) Measurement using 19F NMR Spectroscopy Zhong Wang1, Benjamin F. Jeffries1, Hannah R. Felstead1, Neil J. Wells1, Elisabetta Chiarparin2, Bruno Linclau1 1Chemistry, University of Southampton, 2Medicinal Chemistry, Oncology, IMED Biotech Unit, AstraZeneca A novel and straightforward variation of the shake-flask method was developed for accurate lipophilicity measurement of fluorinated compounds by 19F NMR spectroscopy. Neuroscience Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration Menghon Cheah1, James W. Fawcett1, Melissa R. Andrews2,3 1John van Geest Center for Brain Repair, University of Cambridge, 2School of Medicine, University of St. Andrews, 3Department of Biological Sciences, University of Southampton This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration. Engineering Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems David C. Smith1, Joseph H. Spencer1, Jeremy Sloan2, Liam P. McDonnell1, Harrison Trewhitt2, Reza J. Kashtiban2, Eric Faulques3 1School of Physics and Astronomy, University of Southampton, 2Department of Physics, University of Warwick, 3Institut des Matériaux Jean Rouxel, University of Nantes CNRS The paper describes a method for producing extreme nanowires by melt infiltration into carbon nanotubes and how 1D systems may be characterized and investigated using Resonance Raman Spectroscopy to determine vibrational and optical excitation energies. Medicine Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome Alicja M. Baczynska1,2, Sarah Shaw3, Helen C. Roberts1,2,3,5, Cyrus Cooper2,3,4, Avan Aihie Sayer1,2,3,5,6, Harnish P. Patel1,2,3 1Academic Geriatric Medicine, University of Southampton, University Hospital Southampton, 2National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust, 3MRC Lifecourse Epidemiology Unit, University of Southampton, 4National Institute for Health Research Musculoskeletal Biomedical Research Unit, University of Oxford, 5National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care, 6Newcastle University Institute of Ageing and Institute of Health and Society, Newcastle University This video demonstrates the technique of percutaneous muscle biopsy of the human vastus lateralis using the Weil-Blakesley conchotome. Medicine Measurement of Dynamic Scapular Kinematics Using an Acromion Marker Cluster to Minimize Skin Movement Artifact Martin B. Warner1, Paul H. Chappell2, Maria J. Stokes1 1Faculty of Health Sciences, University of Southampton, 2Electronics and Computer Sciences, University of Southampton This report presents details of how to adopt the acromion marker cluster method of obtaining scapular kinematics when using a passive marker motion capture device. As has been described in the literature, this method provides a robust, non-invasive, three-dimensional, dynamic and valid measurement of scapular kinematics, minimizing skin movement artifact. Neuroscience Preparation of Neuronal Co-cultures with Single Cell Precision Ngoc-Duy Dinh1, Ya-Yu Chiang1,2, Heike Hardelauf1, Sarah Waide1, Dirk Janasek1, Jonathan West1,3 1Leibniz-Institut für Analytische Wissenschaften, ISAS, 2Department of Biochemical Engineering, University College London, 3Institute for Life Sciences, University of Southampton Protocols for single neuron microfluidic arraying and water masking for the in-chip plasma patterning of biomaterial coatings are described. Highly interconnected co-cultures can be prepared using minimal cell inputs. Chemistry The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy Helena L. Fisk1, Annette L. West1, Caroline E. Childs1, Graham C. Burdge1, Philip C. Calder1 1Human Development & Health Academic Unit, Faculty of Medicine, University of Southampton Pregnancy leads to significant changes to the fatty acid composition of maternal tissues. Lipid profiles can be obtained via gas chromatography to allow identification and quantification of fatty acids in individual lipid classes among rats fed various high and low fat diets during pregnancy.
