University of Szeged 4 articles published in JoVE Biology Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage Vasiliki Pantazi*1, Ivett Berzsenyi*1, Barbara N. Borsos1, Tibor Pankotai1 1Institute of Pathology, Faculty of Medicine, University of Szeged This article introduces essential steps of immunostaining and chromatin immunoprecipitation. These protocols are commonly used to study DNA damage-related cellular processes and to visualize and quantify the recruitment of proteins implicated in DNA repair. Behavior Lower-Limb Biomechanical Characteristics Associated with Unplanned Gait Termination Under Different Walking Speeds Huiyu Zhou1,2, Xuanzhen Cen1, Yang Song3, Ukadike C. Ugbolue2, Yaodong Gu1 1Faculty of Sports Science, Ningbo University, 2School of Health and Life Sciences, University of the West of Scotland, 3Faculty of Engineering, University of Szeged This study compared the biomechanical characteristics of the lower extremity during unplanned gait termination under different walking speeds. The lower-limb kinematic and kinetic data from fifteen subjects with normal and fast walking speeds were collected using a motion analysis system and plantar pressure platform. Neuroscience Electrophoretic Delivery of γ-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice Andrea Slezia*1,5, Christopher M. Proctor*2,3, Attila Kaszas1,4, George G. Malliaras2,3, Adam Williamson1,5 1Aix Marseille Université, Institut de Neurosciences des Systèmes (INS), 2Electrical Engineering Division, University of Cambridge, 3Department of Bioelectronics, Centre Microélectronique de Provence - Ecole Nationale Supérieure des Mines de Saint-Étienne (CMP-EMSE), 4Institut de Neurosciences de la Timone, Centre National de la Recherche Scientifique (CNRS) UMR 7289 & Aix- Marseille Université, 5Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged The challenge of epilepsy research is to develop novel treatments for patients where classical therapy is inadequate. Using a new protocol—with the help of an implantable drug delivery system—we are able to control seizures in anesthetized mice by the electrophoretic delivery of GABA into the epileptic focus. Neuroscience Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats Gábor Kozák1, Tamás Földi1, Antal Berényi1,2 1MTA-SZTE "Momentum" Oscillatory Neuronal Networks Research Group, Department of Physiology, University of Szeged, 2New York University Neuroscience Institute, New York University This detailed protocol describes transcranial stimulation electrode placement on the temporal bone in order to investigate the short- and long-term effects of transcranial electrical stimulation in freely moving rats.
