National Food Innovation Hub 1 article published in JoVE Bioengineering Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids Irina A. Okkelman*1, Chris Vercruysse*1, Alina V. Kondrashina2, Sergey M. Borisov3, Ruslan I. Dmitriev1 1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.
