Center for Nanotechnology Innovation 2 articles published in JoVE Biology Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy Gianmarco Ferri1, Fabio Azzarello1, Francesca D'Autilia2, Francesco Cardarelli1 1NEST Laboratory, Scuola Normale Superiore, 2Center for Nanotechnology Innovation@NEST CNI@NEST Imaging-derived mean square displacement (iMSD) analysis is applied to macropinosomes to highlight their intrinsic time-evolving nature in terms of structural and dynamic properties. Macropinosomes are then compared to insulin secretory granules (ISGs) as a reference for subcellular structures with time-invariant average structural/dynamic properties. Chemistry Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors Lorenzo Albertazzi1, Barbara Storti2, Marco Brondi2, Sebastian Sulis Sato2, Gian Michele Ratto3, Giovanni Signore4, Fabio Beltram2 1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry, Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST, Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST, Center for Nanotechnology Innovation Fluorescence sensors are powerful tools in life science. Here we describe a methodology to synthesize and use dendrimer-based fluorescent sensors to measure pH in living cells and in vivo. The dendritic scaffold enhances the properties of conjugated fluorescent dyes leading to improved sensing properties.
