Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER) 1 article published in JoVE Immunology and Infection Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ Melissa Berni1, Stefano Pongolini1, Martina Tambassi1 1 Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
