Radboud University 7 articles published in JoVE Behavior A Within-Subject Experimental Design using an Object Location Task in Rats Gülberk Bayraktar*1,2, Kristoffer Højgaard*1,3, Luc Nijssen1,4, Tomonori Takeuchi1,5 1Danish Research Institute of Translational Neuroscience (DANDRITE), Department of Biomedicine, Aarhus University, 2Universitätsklinikum Würzburg, Institut für Klinische Neurobiologie, 3Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, 4Faculty of Science, Radboud University, 5Center for Proteins in Memory - PROMEMO, Danish National Research Foundation, Department of Biomedicine, Aarhus University This protocol provides detailed steps for an object location task with four repetitions using the same cohort of rats. Weak and strong encoding can produce short- and long-term memories. The flexibility of the protocol with repetition can be beneficial for studies involving surgical operations by saving time and labor. Genetics Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis Jos G.A. Smits*1, Jieqiong Qu*1, Hanna Niehues2, Huiqing Zhou1,3 1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis. Bioengineering A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression Ardjan J. van der Linden1, Maaruthy Yelleswarapu2, Pascal A. Pieters1, Zoe Swank3, Wilhelm T. S. Huck2, Sebastian J. Maerkl3, Tom F. A. de Greef1,2 1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL) The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided. Engineering In Situ Monitoring of the Accelerated Performance Degradation of Solar Cells and Modules: A Case Study for Cu(In,Ga)Se2 Solar Cells Mirjam Theelen1, Klaas Bakker1, Henk Steijvers1, Stefan Roest2, Peter Hielkema3, Nicolas Barreau4, Erik Haverkamp5,6 1TNO Solliance, Thin Film Technology, 2Eternal Sun, 3Hielkema Testequipment, 4Institut des Matériaux Jean Rouxel (IMN)-UMR 6502, Université de Nantes, CNRS, 5ReRa Solutions BV, 6Institute of Molecules and Materials, Radboud University Two 'Combined Stress test with in situ measurement' setups, which allow real-time monitoring of accelerated degradation of solar cells and modules, were designed and constructed. These setups allow the simultaneous use of humidity, temperature, electrical biases, and illumination as independently controlled stress factors. The setups and various experiments executed are presented. Neuroscience Drosophila Courtship Conditioning As a Measure of Learning and Memory Tom S. Koemans1,2,3, Cornelia Oppitz4, Rogier A. T. Donders5, Hans van Bokhoven1,3, Annette Schenck1,3, Krystyna Keleman6, Jamie M. Kramer7,8 1Department of Human Genetics, Radboud University Medical Center, 2Radboud Institute of Molecular Life Sciences, Radboud University, 3Donders Institute for Brain, Cognition, and Behaviour, Centre for Neuroscience, Radboud University, 4Research Institute of Molecular Pathology, Vienna, Austria, 5Department for Health Evidence, Radboud University Medical Center, 6Janelia Research Campus, Howard Hughes Medical Institute, 7Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Western University, 8Department of Biology, Faculty of Science, Western University This protocol describes a Drosophila learning and memory assay called courtship conditioning. This classic assay is based on a reduction of male courtship behavior after sexual rejection by a non-receptive premated female. This natural form of behavioral plasticity can be used to test learning, short-term memory, and long-term memory. Developmental Biology Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays Monica Frega*1,2, Sebastianus H. C. van Gestel*3, Katrin Linda2,3, Jori van der Raadt3, Jason Keller1,2, Jon-Ruben Van Rhijn1,2, Dirk Schubert1,2, Cornelis A. Albers2,3,4, Nael Nadif Kasri1,2,3 1Department of Cognitive Neurosciences, Radboudumc, 2Donders Institute for Brain, Cognition and Behaviour, Radboud University, 3Department of Human Genetics, Radboudumc, 4Department of Molecular Developmental Biology, Radboud University We modify and implement a previously published protocol describing the rapid, reproducible, and efficient differentiation of human induced Pluripotent Stem Cells (hiPSCs) into excitatory cortical neurons12. Specifically, our modification allows for control of neuronal cell density and use on micro-electrode arrays to measure electrophysiological properties at the network level. Behavior The Resident-intruder Paradigm: A Standardized Test for Aggression, Violence and Social Stress Jaap M. Koolhaas1, Caroline M. Coppens1, Sietse F. de Boer1, Bauke Buwalda1, Peter Meerlo1, Paul J.A. Timmermans2 1Department of Behavioral Physiology, Center for Behavior and Neurosciences, University Groningen, 2Radboud University Nijmegen This video shows the resident-intruder paradigm in rats. This test is a standardized method to measure offensive aggression, defensive behavior and violence in a semi-natural setting. The use of the paradigm for social stress experiments is explained as well.
