Interuniversity Cardiology Institutes of the Netherlands (ICIN) 2 articles published in JoVE Medicine Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction Guilielmus H.J.M. Ellenbroek1, Gerardus P.J. van Hout1,2, Leo Timmers1,2, Pieter A. Doevendans2,4, Gerard Pasterkamp1,3, Imo E. Hoefer1,3 1Department of Experimental Cardiology, University Medical Center Utrecht, 2Department of Cardiology, University Medical Center Utrecht, 3Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, 4Interuniversity Cardiology Institutes of the Netherlands (ICIN) Reliable and accurate outcome assessment is the key for translation of preclinical therapies into clinical treatment. The current paper describes how to assess three clinically relevant primary outcome parameters of cardiac performance and damage in a pig acute myocardial infarction model. Medicine Myocardial Infarction and Functional Outcome Assessment in Pigs Stefan Koudstaal1,2, Sanne J. Jansen of Lorkeers1, Johannes M.I.H. Gho1, Gerardus P.J van Hout1, Marlijn S. Jansen1, Paul F. Gründeman1, Gerard Pasterkamp1,2, Pieter A. Doevendans1,2, Imo E. Hoefer*1, Steven A.J. Chamuleau*1 1Department of Cardiology, Division Heart and Lungs, University Medical Center Utrecht, 2Interuniversity Cardiology Institute of the Netherlands This protocol describes the porcine myocardial infarction (MI) model using a 90 min closed-chest coronary balloon occlusion of the left anterior descending artery (LAD), followed by reperfusion. Furthermore, the protocol for several outcome parameters, such as cardiac function, hemodynamics, microvascular resistance, and infarct size, are also presented.
