UMC Utrecht 3 articles published in JoVE Neuroscience Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats Elisabeth Maria Brakkee1, Erick DeVinney2, Niels Eijkelkamp3, J. Henk Coert1 1Department of Plastic and Reconstructive Surgery, University Medical Center Utrecht, Utrecht University, 2Axogen, 3Center for Translational Immunology, University Medical Center Utrecht, Utrecht University This protocol describes the tibial neuroma transposition model, which entails a lesion of the tibial nerve with subsequent transposition of the proximal nerve end toward a subcutaneous pretibial or lateral position. Behavioral testing of neuroma pain and plantar hyperalgesia is quantified using Von Frey monofilaments. Neuroscience Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins Cristina Melero*1, Aljona Kolmogorova*1, Paul Atherton1, Brian Derby2, Adam Reid3,4, Karin Jansen5, Christoph Ballestrem1 1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research, The University of Manchester, 2School of Materials, The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, 5Department of Pathology, UMC Utrecht Our overall aim is to understand how cells sense extracellular cues that lead to directed axonal growth. Here, we describe the methodology of Light-Induced Molecular Adsorption of Proteins, used to produce defined micro-patterns of extracellular matrix components in order to study specific events that govern axon outgrowth and pathfinding. Immunology and Infection Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection Devanjali Dutta1, Inha Heo1,3, Roberta O'Connor2 1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), UMC Utrecht, 2Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, 3Janssen Pharmaceutica We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.
