Stockholm University 4 articles published in JoVE Bioengineering 3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds Sahar Sultan1, Aji P. Mathew1 1Department of Materials and Environmental Chemistry, Stockholm University The three critical steps of this protocol are i) developing the right composition and consistency of the cellulose hydrogel ink, ii) 3D printing of scaffolds into various pore structures with good shape fidelity and dimensions and iii) demonstration of the mechanical properties in simulated body conditions for cartilage regeneration. Biochemistry Rapid Isolation of the Mitoribosome from HEK Cells Shintaro Aibara1, Juni Andréll1, Vivek Singh1, Alexey Amunts1 1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes. Chemistry Characterization, Quantification and Compound-specific Isotopic Analysis of Pyrogenic Carbon Using Benzene Polycarboxylic Acids (BPCA) Daniel B. Wiedemeier1, Susan Q. Lang2, Merle Gierga3, Samuel Abiven1, Stefano M. Bernasconi3, Gretchen L. Früh-Green3, Irka Hajdas4, Ulrich M. Hanke1, Michael D. Hilf1, Cameron P. McIntyre4, Maximilian P. W. Scheider1, Rienk H. Smittenberg5, Lukas Wacker4, Guido L. B. Wiesenberg1, Michael W. I. Schmidt1 1Department of Geography, University of Zurich, 2Department of Earth and Ocean Sciences, University of South Carolina, 3Department of Earth Sciences, ETH Zurich, 4Laboratory of Ion Beam Physics, ETH Zurich, 5Department of Geological Sciences, Stockholm University We present the benzene polycarboxylic acid (BPCA) method for assessing pyrogenic carbon (PyC) in the environment. The compound-specific approach uniquely provides simultaneous information about the characteristics, quantity and isotopic composition (13C and 14C) of PyC. Developmental Biology Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos Giselbert Hauptmann1, Iris Söll1, Robert Krautz1, Ulrich Theopold1 1Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.
