AstraZeneca Global Medicines Development 1 article published in JoVE Immunology and Infection A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide Abraham B. Roos1, Tove Berg1, Kerstin M. Ahlgren1, Johan Grunewald1, Magnus Nord1,2 1Department of Medicine, Solna and CMM, Respiratory Medicine Unit, Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety, AstraZeneca Global Medicines Development We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.
