Bielefeld University View Institution's Website 6 articles published in JoVE Biochemistry Förster Resonance Energy Transfer Measurements in Living Plant Cells Sonja Michèle Schmidtpott1, Thorsten Seidel1 1Dynamic Cell Imaging, Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation. Behavior Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects Camilo Rodriguez Ronderos1, Katja Münster1, Ernesto Guerra2, Helene Kreysa3, Alba Rodríguez1,4, Julia Kröger4, Thomas Kluth4, Michele Burigo4, Dato Abashidze5, Eva Nunnemann4, Pia Knoeferle1,6,7 1Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, 2Center for Advanced Research in Education – CIAE, Universidad de Chile, 3Institute of Psychology, Friedrich Schiller University Jena, 4Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, 5Department of Education, Concordia University, 6Berlin School of Mind and Brain, 7Einstein Center for Neurosciences Berlin The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration. Chemistry Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry Jens Sproß1 1Department of Chemistry, Institute of Organic Chemistry, Bielefeld University This video presents a protocol for the mass spectrometrical analysis of volatile and oxidation sensitive compounds using electron impact ionization. The presented technique is especially of interest for inorganic chemists, working with metal organyls, silanes, or phosphanes which have to be handled using inert conditions, such as the Schlenk technique. Engineering Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes Nils Hansen1, Scott A. Skeen1, Hope A. Michelsen1, Kevin R. Wilson2, Katharina Kohse-Höinghaus3 1Combustion Research Facility, Sandia National Laboratories, 2Chemical Sciences Division, Advanced Light Source, Lawrence Berkeley National Laboratory, 3Physikalische Chemie I, Universität Bielefeld Gas sampling from laboratory-scale flames with online analysis of all species by mass spectrometry is a powerful method to investigate the complex mixture of chemical compounds occurring during combustion processes. Coupled with tunable soft ionization via synchrotron-generated vacuum-ultraviolet radiation, this technique provides isomer-resolved information and potentially fragment-free mass spectra. Neuroscience Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis Darius Widera1, Janine Müller2, Yvonne Imielski2, Peter Heimann1, Christian Kaltschmidt1, Barbara Kaltschmidt2 1Cell Biology, University of Bielefeld, 2Molecular Neurobiology, University of Bielefeld Methods for the manipulation and analysis of NF-κB-dependent adult hippocampal neurogenesis are described. A detailed protocol is presented for a dentate gyrus-dependent behavioral test (termed the spatial pattern separation-Barnes maze) for the investigation of cognitive outcome in mice. This technique should also help enable investigations in other experimental settings. Neuroscience Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.) Samir Mujagić1, Simon Michael Würth1, Sven Hellbach1, Volker Dürr1 1Biological Cybernetics, CITEC - Cognitive Interaction Technology - Center of Excellence, Bielefeld University In this protocol we show how to condition harnessed honey bees to tactile stimuli and introduce a 2D motion capture technique for analyzing the kinematics of fine-scale antennal sampling pattern.