Max-Delbrück-Center for Molecular Medicine

7 articles published in JoVE

Monitoring Dendritic Cell Migration using ¹⁹F / ¹H Magnetic Resonance Imaging

JoVE 50251 3/20/2013

Helmar Waiczies^1,2, Martin Guenther^1,2, Julia Skodowski^1,2, Stefano Lepore^1,2, Andreas Pohlmann², Thoralf Niendorf^1,2, Sonia Waiczies^1,2

¹Experimental and Clinical Research Center, A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, ²Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (¹⁹F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with ¹⁹F/¹H MRI and ¹⁹F MRS.

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes

JoVE 50145 3/12/2013

Clemens Köhncke^1,2, Ulrike Lisewski¹, Leonhard Schleußner¹, Carolin Gaertner¹, Saskia Reichert¹, Torsten K. Roepke^1,3

¹Experimental and Clinical Research Center (ECRC), Charité Medical Faculty and Max-Delbrück Center for Molecular Medicine (MDC), ²Medical Department, Division of Cardiology, Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, ³Medical Department, Division of Cardiology and Angiology, Campus Mitte, Charité - Universitätsmedizin Berlin

Kv channel dysfunction is associated with cardiac arrhythmias. In order to study the molecular mechanisms that lead to such arrhythmias we utilize a systematic protocol for isolation of atrial and ventricular cardiomyocytes from Kv channel ancillary subunit knockout mice. Isolated cardiomyocytes can then immediately be used for cellular electrophysiological studies, biochemical or immunofluorescence (IF) assays.

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

JoVE 4351 1/30/2013

Cécile Otten, Salim Abdelilah-Seyfried

Max Delbrück Center for Molecular Medicine

The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

JoVE 4350 9/28/2012

Verónica A. Lombardo, Anje Sporbert, Salim Abdelilah-Seyfried

Max Delbrück Center for Molecular Medicine

Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.

A System for ex vivo Culturing of Embryonic Pancreas

JoVE 3979 8/27/2012

Kristin M. Petzold, Francesca M. Spagnoli

Molecular and Cellular Basis of Embryonic Development, Max-Delbrück-Center for Molecular Medicine

Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.

DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

JoVE 3667 12/13/2011

Hannes Schmidt, Fritz G. Rathjen

Developmental Neurobiology, Max Delbrück Center for Molecular Medicine

The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

JoVE 2034 7/02/2010

Markus Hafner¹, Markus Landthaler², Lukas Burger³, Mohsen Khorshid³, Jean Hausser⁴, Philipp Berninger⁴, Andrea Rothballer¹, Manuel Ascano¹, Anna-Carina Jungkamp², Mathias Munschauer², Alexander Ulrich¹, Greg S. Wardle¹, Scott Dewell⁵, Mihaela Zavolan³, Thomas Tuschl¹

¹Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, ²Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, ³Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), ⁴Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), ⁵Genomics Resource Center, Rockefeller University

RNA transcripts are subject to extensive posttranscriptional regulation that is mediated by a multitude of trans-acting RNA-binding proteins (RBPs). Here we present a generalizable method to identify precisely and on a transcriptome-wide scale the RNA binding sites of RBPs.