Universitat Autonoma de Barcelona View Institution's Website 10 articles published in JoVE Immunology and Infection Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography Sandra Guallar-Garrido1, Marina Luquin1, Esther Julián1 1Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona A protocol is presented to extract the total lipid content of the cell wall of a wide range of mycobacteria. Moreover, extraction and analytical protocols of the different types of mycolic acids are shown. A thin-layer chromatographic protocol to monitor these mycobacterial compounds is also provided. Cancer Research In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells Joan Repullés1,2, Mariona Terradas1,3,4, Gemma Fuster5,6,7, Anna Genescà1, Teresa Anglada1 1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described. Bioengineering Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential Aida Llucià-Valldeperas1,2, Ramon Bragós3, Antoni Bayés-Genís1,4,5,6 1Insuficiencia Cardiaca y Regeneración Cardiaca (ICREC) Research Program, Health Science Research Institute Germans Trias i Pujol, 2Amsterdam Universitair Medisch Centrum (UMC), Vrije Universiteit Amsterdam, Pulmonology and Physiology, Amsterdam Cardiovascular Sciences, 3Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya, 4Cardiology Service, Germans Trias i Pujol University Hospital, 5Department of Medicine, Universitat Autònoma de Barcelona, 6Centro de Investigación Biomédica en Red (CIBER) Cardiovascular, Instituto de Salud Carlos III Here we present a protocol for training a cell population using electrical and mechanical stimuli emulating cardiac physiology. This electromechanical stimulation enhances the cardiomyogenic potential of the treated cells and is a promising strategy for further cell therapy, disease modeling, and drug screening. Immunology and Infection Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds Jie Ji1,2, Rosemary Thwaite1,2, Nerea Roher1,2 1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona The protocol describes intubating adult zebrafish with a biologic; then dissecting and preparing the intestine for cytometry, confocal microscopy and qPCR. This method allows administration of bioactive compounds to monitor intestinal uptake and the local immune stimulus evoked. It is relevant for testing the intestinal dynamics of oral prophylactics. Biochemistry Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast Anita Carija1, Salvador Ventura1, Susanna Navarro1 1Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide. Behavior Using Electroencephalography Measurements and High-quality Video Recording for Analyzing Visual Perception of Media Content Miguel Ángel Martín-Pascual1,2, Celia Andreu-Sánchez1, José María Delgado-García3, Agnès Gruart3 1Neuro-Com Research Group, Universitat Autónoma de Barcelona, 2Instituto RTVE, Corporación Radio Televisión Española, 3Division of Neuroscience, Pablo de Olavide University We present detection, acquisition, and analysis of eyeblink rates while watching media content. Developmental Biology Derivation of Stem Cell Lines from Mouse Preimplantation Embryos Marta Vila-Cejudo1, Elena Ibañez1, Josep Santalo1 1Unitat de Biologia Cellular, Facultat de Biociències, Universitat Autònoma de Barcelona This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage. Environment How to Create Conditioned Taste Aversion for Grazing Ground Covers in Woody Crops with Small Ruminants Carmen L. Manuelian1, Elena Albanell1, Maristela Rovai1,2, Gerardo Caja1 1Group of Ruminant Research (G2R), Department of Animal and Food Science, Universitat Autònoma de Barcelona, 2Dairy Science Department, South Dakota State University Feeding behavior preferences in livestock can be modified to implement a grazing management plan in woody crops. Here, we present a protocol to show a lithium chloride dose after eating a new plant that induces conditioned taste aversion. Biology Fluorescence in situ hybridization (FISH) Protocol in Human Sperm Zaida Sarrate1, Ester Anton1 1Unitat de Biologia Cel·lular (Facultat de Biociències), Universitat Autónoma de Barcelona This video-article describes, step by step, how to process a semen sample to achieve good-quality fluorescence in situ hybridization on human spermatozoa. Preparations obtained can be used for aneuploidy screening in the context of clinical diagnosis. Biology Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos Nuno Costa-Borges1, Sheyla González1, Elena Ibáñez1, Josep Santaló1 1Unitat Biologia Cellular (Facultat de Biociències), Universitat Autonoma de Barcelona In this video-article we present a step-by-step demonstration on how to collect and cryopreserve hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.
