Barcelona Centre for International Health Research View Institution's Website 1 article published in JoVE Immunology and Infection Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow Mireia Ferrer*1, Lorena Martin-Jaular*1, Maria Calvo2, Hernando A. del Portillo1,3 1Department of poverty related diseases, Barcelona Centre for International Health Research, 2Confocal Microscopy Unit, University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA) We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.