Instituto Gulbenkian de Ciência View Institution's Website 1 article published in JoVE Immunology and Infection Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy Susana S. Caetano1, Tatiana Teixeira1, Carlos E. Tadokoro1 1Laboratory of Immune Regulation, Instituto Gulbenkian de Ciência We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.