Vancouver Hospital and Health Sciences Centre 1 article published in JoVE Medicine Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice Kyle Peake1, John Manning1, Coral-Ann Lewis1,2, Christine Barr1, Fabio Rossi2, Charles Krieger1,3 1Department of Biomedical Physiology and Kinesiology, Simon Fraser University, 2The Biomedical Research Centre, University of British Columbia, 3Division of Neurology, Department of Medicine, Neuromuscular Disease Unit, VHHSC We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.
