IUCPQ Research Center 1 article published in JoVE Developmental Biology Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures Julie Lessard1, Julie Anne Côté1, Marc Lapointe1, Mélissa Pelletier2, Mélanie Nadeau1, Simon Marceau1, Laurent Biertho1, André Tchernof1,2,3 1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.