Institut de Recherches Cliniques de Montreal (IRCM) 3 articles published in JoVE Genetics Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells Francis Migneault1,2,3, Frédéric Gagnon2,3, Emmanuelle Brochiero1,3, Yves Berthiaume2,3, André Dagenais2,3 1 Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique. Immunology and Infection Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System. Mariana G. Bego1, Édouard A. Côté1, Éric A. Cohen1,2 1Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Microbiology, Infectiology, and Immunology, Université de Montréal Unlike cell-free HIV-1 particles, infected CD4+ T cells are effectively sensed by plasmacytoid dendritic cells (pDCs). This manuscript describes a method where peripheral blood mononuclear cells (PBMCs) or isolated pDCs are co-cultured with HIV-1 infected T cells to evaluate innate sensing by pDCs as assessed by release of type-I IFN. Neuroscience Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation Roberta Haddad-Tóvolli*1, Nora-Emöke Szabó*2, Xunlei Zhou1, Gonzalo Alvarez-Bolado1 1Institute of Anatomy and Cell Biology, University of Heidelberg, 2Institut de recherches cliniques de Montreal Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
