London Regional Cancer Program 1 article published in JoVE Cancer Research Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples Vasudeva Bhat1,2, Cory Lefebvre1,2, David Goodale1, Mauricio Rodriguez-Torres1,2, Alison L. Allan1,2,3,4 1London Regional Cancer Program, 2Department of Anatomy & Cell Biology, Western University, 3Department of Oncology, Western University, 4Lawson Health Research Institute This experimental protocol describes the isolation of BCSCs from breast cancer cell and tissue samples as well as the in vitro and in vivo assays that can be used to assess BCSC phenotype and function.
