The University of British Columbia 1 article published in JoVE Biology Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay Scott Minh An*1,2, Seong Ho Kim*1, Vanessa J. White*1,2, Adam B. Yasunaga*1,2, Kathleen M. McMahon2, Yousif Murad1,3, Isaac T. S. Li1,2 1Department of Chemistry, The University of British Columbia, 2Biochemistry and Molecular Biology, The University of British Columbia, 3Faculty of Medicine, The University of British Columbia This protocol presents the experimental procedures to perform the adhesion footprint assay to image the adhesion events during fast cell rolling adhesion.