Keenan Research Centre for Biomedical Science of St. Michael’s Hospital 1 article published in JoVE Immunology and Infection Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune Encephalomyelitis Carmen C. Ucciferri1, Annette Gower2, Nuria Alvarez-Sanchez1,2, Heather Whetstone3, Valeria Ramaglia1, Jennifer L. Gommerman1, Koroboshka Brand-Arzamendi2, Raphael Schneider2,4, Shannon E Dunn1,2,5 1Department of Immunology, University of Toronto, 2Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, 3Sickkids Research Institute, The Hospital for Sick Children, 4Women’s College Research Institute, Women’s College Hospital Experimental autoimmune encephalomyelitis (EAE) serves as an animal model of multiple sclerosis. This article describes an approach for scoring spinal cord inflammation, demyelination, and axonal injury in EAE. Additionally, a method to quantify soluble neurofilament light levels in the mice serum is presented, facilitating the assessment of axonal injury in live mice.
