Center for Scientific Research and Higher Education at Ensenada 2 articles published in JoVE Biology Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera) Edward M. Hsieh1, Jimena Carrillo-Tripp2, Adam G. Dolezal1 1Department of Entomology, University of Illinois, 2Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE) Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay. Cancer Research Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry Cinthia N. Cuero1, Brenda Iduarte1, Patricia Juárez1 1Biomedical Innovation Department, Center for Scientific Research and Higher Education at Ensenada The ex vivo culture of bone explants can be a valuable tool for the study of bone physiology and the potential evaluation of drugs in bone remodeling and bone diseases. The presented protocol describes the preparation and culture of calvarias isolated from newborn mice skulls, as well as its applications.
