Universidad del Valle de Mexico 1 article published in JoVE Immunology and Infection Quantitation of Rabies Virus in Various Bovine Brain Structures Edith Rojas-Anaya1, Daniela Anaya-Razo2, Isabel Bárcenas-Reyes3, Elizabeth Loza-Rubio1 1Centro Nacional de Investigación Disciplinaria en Microbiología Animal (CENID-MA), INIFAP, 2Campus Toluca, Universidad del Valle de México, 3Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro This protocol presents a qRT-PCR-based approach for determining the rabies virus nucleoprotein (N) gene copy number within various bovine brain anatomical structures using in vitro transcription.
