Instituto Mexicano del Seguro Social 2 articles published in JoVE Biology Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats Aymé Oliva Cárdenas1,2, Blanca C. Zamora-Rodríguez2, Kevin A. Batalla-García2, Alejandro Ávalos-Rodríguez3, Alejandra Contreras-Ramos4, Clara Ortega-Camarillo2 1Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, 2Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI, Instituto Mexicano de Seguro Social, 3Department of Agricultural and Animal Production, Division of Biological and Health Science, Universidad Autónoma Metropolitana-Xochimilco, 4Molecular Biology Research Laboratory, Center for Congenital Malformations, Children Hospital of Mexico Federico Gomez (HIMFG) This protocol describes a methodology for isolating and identifying adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats. Medicine A Modified Sonographic Algorithm for Image Acquisition in Life-Threatening Emergencies in the Critically Ill Newborn Daniel Ibarra-Ríos1, Eunice Valeria Serpa-Maldonado1, José Guadalupe Mantilla-Uresti1, Rogelio Guillén-Torres1, Naomi Aguilar-Martínez2, Alejandra Sánchez-Cruz3, Deneb Algedi Morales-Barquet4, Rosario Becerra-Becerra5, Horacio Márquez-González6 1Hemodynamic Consultation and Ultrasound in the Critically Ill Neonate, Hospital Infantil de México Federico Gómez (HIMFG), 2Neonatology Department, HIMFG, 3Neonatal Intensive Care Unit, Hospital de Ginecología y Obstetricia Número 4, Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, 4Head of Intermediate Care, Instituto Nacional de Perinatología, 5Head of the Echocardiography Lab, HIMFG, 6Clinical Investigation Department, HIMFG Here, we present a protocol that can be applied in the neonatal intensive care unit and the delivery room in relation to three scenarios: cardiac arrest, hemodynamic deterioration, or respiratory decompensation. This protocol can be performed with a state-of-the-art ultrasound machine or an affordable handheld device; an image acquisition protocol is carefully detailed.
