University of Arizona College of Medicine Tucson 1 article published in JoVE Biology Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma Md A. Hakim1, Paulo W. Pires2, Erik J. Behringer1 1Department of Basic Sciences, Loma Linda University, 2Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center, University of Arizona College of Medicine Tucson Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.
