California State University, Chico 1 article published in JoVE Developmental Biology Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish Kristen F. Rueb1, David L. Stachura1 1Department of Biological Sciences, California State University, Chico This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.