Yale School of Medicine 3 articles published in JoVE Biology Generation and Manipulation of Rat Intestinal Organoids Eleanor Zagoren*1, Anderson K. Santos*1,2, Nadia A. Ameen3,4, Kaelyn Sumigray1,5,6 1Department of Genetics, Yale School of Medicine, 2Department of Pediatrics, Yale School of Medicine, 3Department of Pediatrics/Gastroenterology and Hepatology, Yale School of Medicine, 4Department of Cellular and Molecular Physiology, Yale School of Medicine, 5Yale Stem Cell Center, Yale School of Medicine, 6Yale Cancer Center, Yale School of Medicine Here, we present a protocol to generate rat intestinal organoids and use them in several downstream applications. Rats are often a preferred preclinical model, and the robust intestinal organoid system fills the need for an in vitro system to accompany in vivo studies. Biochemistry Self-Assembly of Microtubule Tactoids Prashali Chauhan1, Sumon Sahu1,2, Niaz Goodbee1, Sophia Martin1, Hong Beom Lee1, Ruell Branch1, Jennifer M. Schwarz1, Jennifer L. Ross1 1Physics Department, Syracuse University, 2Department of Molecular Biophysics and Biochemistry, Yale School of Medicine This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent. Bioengineering Engineered Lung Tissues Prepared from Decellularized Lung Slices Katherine L. Leiby1,2, Ronald Ng1, Stuart G. Campbell1,3, Laura E. Niklason1,4 1Department of Biomedical Engineering, Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology, Yale School of Medicine, 4Department of Anesthesiology, Yale School of Medicine This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.
