Fraunhofer USA Center for Molecular Biotechnology 1 article published in JoVE Biology Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana Moneim Shamloul1, Jason Trusa1, Vadim Mett1, Vidadi Yusibov1 1Fraunhofer USA Center for Molecular Biotechnology Transient protein production in Nicotiana plants based on vacuum infiltration with Agrobacteria carrying launch vectors (Tobacco mosaic virus-based) is a rapid and economic approach to produce vaccine antigens and therapeutic proteins. We simplified the procedure and improved target accumulation by optimizing conditions of bacteria cultivation, selecting host species, and co-introducing RNA silencing suppressors.
