Foundation for Applied Molecular Evolution (FfAME) 1 article published in JoVE Biochemistry Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1 Ozlem Yaren1, Barry W. Alto2, Kevin M. Bradley3, Patricia Moussatche3, Lyudmyla Glushakova3, Steven A. Benner1,3 1Foundation for Applied Molecular Evolution (FfAME), 2Florida Medical Entomology Laboratory, University of Florida, 3Firebird Biomolecular Sciences LLC Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.
