Georgia Institute of Technology and Emory University 5 articles published in JoVE Neuroscience Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury Rowan O. Brothers*1, Sara Bitarafan*2,3, Alyssa F. Pybus1,3, Levi B. Wood*1,2,3, Erin M. Buckley*1,4,5 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 3Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 4Department of Pediatrics, Emory University School of Medicine, 5 This protocol presents methods to characterize the neuroinflammatory and hemodynamic response to mild traumatic brain injury and to integrate these data as part of a multivariate systems analysis using partial least squares regression. Biology Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments Sandeep Kumar*1, Aitor Andueza*1, Nicolas Villa-Roel1, Juyoung Kim1, Dong-Won Kang1, Hanjoong Jo1,2 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments. Bioengineering Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets Andrew Zhao1, Jeungyoon Lee2, Stanislav Emelianov1,3 1Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2School of Electrical Engineering, Georgia Institute of Technology, 3Department of Biomedical Engineering and School of Electrical Engineering, Georgia Institute of Technology and Emory University Optically activated perfluorocarbon nanodroplets show promise in imaging applications outside of the vascular system. This article will demonstrate how to synthesize these particles, crosslink polyacrylamide phantoms, and modulate the droplets acoustically to enhance their signal. Bioengineering DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells Rong Ma1, Anna V. Kellner2, Yuesong Hu1, Brendan R. Deal1, Aaron T. Blanchard2, Khalid Salaita1,2 1Department of Chemistry, Emory University, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time. Bioengineering Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics Brandon Alexander Holt1, Quoc D. Mac1, Gabriel A. Kwong1,2,3,4,5 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech College of Engineering and Emory School of Medicine, 2Parker H. Petit Institute of Bioengineering and Bioscience, 3Institute for Electronics and Nanotechnology, Georgia Tech, 4Integrated Cancer Research Center, Georgia Tech, 5The Georgia Immunoengineering Consortium, Emory University and Georgia Tech Proteases are tightly regulated enzymes involved in fundamental biological processes, and dysregulated protease activity drives progression of complex diseases such as cancer. This method's goal is to create nanosensors that measure protease activity in vivo by producing a cleavage signal that is detectable from host urine and discriminates disease.