University of Iowa Hospitals and Clinics 1 article published in JoVE Medicine Primary Culture of Human Vestibular Schwannomas Nathan M. Schularick1, J. Jason Clark1, Marlan R. Hansen1 1Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics Vestibular Schwannomas (VSs) are non-malignant tumors of Schwann cell (SC) origin, associated with mutations in the NF2 tumor suppressor gene. We report a reproducible, efficient protocol for primary human VS cell culture that allows for molecular and cellular experimental manipulation and analysis and recapitulates the heterogeneous nature of human disease.
