LSU Health Sciences Center 1 article published in JoVE Medicine Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR Marco Pacifici1, Serena Delbue2, Ferdous Kadri1, Francesca Peruzzi1 1Medical School and Stanley S. Scott Cancer Center, LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences, University of Milan We describe a protocol of real time PCR to profile microRNAs in the cerebrospinal fluid (CSF). With the exception of RNA extraction protocols, the procedure can be extended to RNA extracted from other body fluids, cultured cells, or tissue specimens.
