USDA, APHIS, Wildlife Services, Sutton, MA 1 article published in JoVE Immunology and Infection Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test Erin M. Patrick1, Brian M. Bjorklund2, Jordona D. Kirby3, Kathleen M. Nelson4, Richard B. Chipman4, Charles E. Rupprecht5 1Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Knoxville, TN, United States Department of Agriculture (USDA), 2Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Sutton, MA, United States Department of Agriculture (USDA), 3Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Milton, FL, United States Department of Agriculture (USDA), 4Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Concord, NH, United States Department of Agriculture (USDA), 5Lyssa LLC The direct rapid immunohistochemical test (DRIT) offers a World Organization for Animal Health and World Health Organization (OIE/WHO) recognized alternative to the direct fluorescent antibody (DFA) test for rabies diagnosis. This test allows for field-based applications that can be performed in approximately 1 h upon brain impressions using light microscopy.
