University of Massachusetts Chan Medical School 3 articles published in JoVE Biochemistry Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals Tenzin Tseyang*1, Jonathan Valeros*1, Paula Vo*1, Jessica B. Spinelli1,2 1Program in Molecular Medicine, University of Massachusetts Chan Medical School, 2Cancer Center, University of Massachusetts Chan Medical School Here, we present a compilation of assays to directly measure mitochondrial function in mammalian cells independently of their ability to consume molecular oxygen. Neuroscience Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope Jianjun Zhong1,2, Georgia Gunner3, Nils Henninger1, Dorothy P. Schafer3, Daryl A. Bosco1 1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy. Neuroscience Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes Salome Funes1,2, Daryl A. Bosco1 1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School This protocol describes the differentiation process of human induced pluripotent stem cells (iPSCs) into microglia-like cells for in vitro experimentation. We also include a detailed procedure for generating human synaptosomes from iPSC-derived lower motor neurons that can be used as a substrate for in vitro phagocytosis assays using live-cell imaging systems.
