Mercy Health Hauenstein Neuroscience Medical Center 2 articles published in JoVE Neuroscience Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson's Disease Margaret E. Caulfield1, Jennifer A. Stancati1, Kathy Steece-Collier1,2 1Department of Translational Neuroscience, College of Human Medicine, Michigan State University, 2Hauenstein Neuroscience Center, Mercy Health Saint Mary's This article describes methods to induce and evaluate levodopa-induced dyskinesias in a rat model of Parkinson's disease. The protocol offers detailed information regarding the intensity and frequency of a range of dyskinetic behaviors, both dystonic and hyperkinetic, providing a reliable tool to test treatments targeting this unmet medical need. Neuroscience Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo Joseph R. Patterson1, Nicole K. Polinski2, Megan F. Duffy1,3, Christopher J. Kemp1, Kelvin C. Luk4, Laura A. Volpicelli-Daley5, Nicholas M. Kanaan1, Caryl E. Sortwell1,3,6 1Department of Translational Science and Molecular Medicine, Michigan State University, 2 The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.
