Missouri State University 1 article published in JoVE Chemistry Synthesis of Cd-free InP/ZnS Quantum Dots Suitable for Biomedical Applications Matthew A. Ellis1, Giovanna Grandinetti2, Katye M. Fichter1 1Department of Chemistry, Missouri State University, 2Center for Molecular Microscopy, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research In this protocol, the synthesis of Cd-free InP/ZnS quantum dots (QDs) is detailed. InP-based QDs are gaining popularity due to the toxicity of Cd2+ ions that may be released through nanoparticle degradation. After synthesis, QDs are solubilized in water using an amphiphilic polymer for use in biomedical applications.