University of North Dakota School of Medicine and Health Sciences 1 article published in JoVE Cancer Research ATAC-Seq Optimization for Cancer Epigenetics Research Mikhala Cooper*1, Atrayee Ray*1, Atrayee Bhattacharya2, Archana Dhasarathy1, Motoki Takaku1 1University of North Dakota School of Medicine and Health Sciences, 2Dana-Farber Cancer Institute ATAC-seq is a DNA sequencing method that uses the hyperactive mutant transposase, Tn5, to map changes in chromatin accessibility mediated by transcription factors. ATAC-seq enables the discovery of the molecular mechanisms underlying phenotypic alterations in cancer cells. This protocol outlines optimization procedures for ATAC-seq in epithelial cell types, including cancer cells.
