University at Buffalo, State University of New York 4 articles published in JoVE Behavior Using Unidirectional Rotations to Improve Vestibular System Asymmetry in Patients with Vestibular Dysfunction Nayer Rassaian1, Navid G. Sadeghi1, Bardia Sabetazad2, Kathleen M. McNerney3, Robert F. Burkard4, Soroush G. Sadeghi5 1Department of Physiology, Shahid Beheshti University of Medical Sciences and Health Services, 2Audiology and Dizziness Center, Dey General Hospital, 3Department of Speech-Language Pathology, SUNY Buffalo State, 4Department of Rehabilitation Science, School of Public Health and Health Professions, State University of New York at Buffalo, 5Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, State University of New York at Buffalo A new rehabilitation method is presented for rebalancing the vestibular system in patients with asymmetric responses, which consists of unidirectional rotations toward the weaker side. By directly modifying the vestibular pathway rather than enhancing the multisensory aspects of compensation, asymmetry can be normalized within 1-2 sessions and show lasting effects. Medicine Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy Eriko Katsuta1, Masanori Oshi1, Omar M. Rashid2,3,4,5, Kazuaki Takabe1,6,7,8,9,10 1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression. Immunology and Infection Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes Emily A. Clementi*1, Laura R. Marks*1, Hazeline Roche-Håkansson1, Anders P. Håkansson1,2,3 1Department of Microbiology and Immunology, University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York Unlike that seen for eukaryotes, there is a paucity of studies that detail membrane depolarization and ion concentration changes in bacteria, primarily as their small size makes conventional methods of measurement difficult. Here, we detail protocols for monitoring such events in the significant Gram-positive pathogen Streptococcus pneumoniae utilizing fluorescence techniques. Bioengineering One Minute, Sub-One-Watt Photothermal Tumor Ablation Using Porphysomes, Intrinsic Multifunctional Nanovesicles Cheng S. Jin1,2,3, Jonathan F. Lovell4, Gang Zheng1,2,3 1Department of Pharmaceutical Sciences, University of Toronto, 2The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 3Ontario Cancer Institute, Campbell Family Institute For Cancer Research and Techna Institute, 4Department of Biomedical Engineering, University at Buffalo, The State University of New York We developed novel intrinsic multifunctional nanovesicles called porphysomes, which have structure-dependent fluorescence self-quenching and unique photothermal properties, thus functioning as potent photothermal therapy agents. We formulated porphysomes using high pressure extrusion and investigated their photothermal therapy efficacy in a xenograft tumor model.
