Juniata College 3 articles published in JoVE Environment Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures Jeremy R. Chen See1,2, Olivia Wright1, Lavinia V. Unverdorben1,2, Nathan Heibeck1, Stephen M. Techtmann3, Terry C. Hazen4,5, Regina Lamendella1,2 1Department of Biology, Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences, Michigan Technological University, 4Biosciences Division, Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering, University of Tennessee Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities. Neuroscience Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle Maria Cano Garcia1, Steven C. Nesbit1, Chi C. Le2, James R. Dearworth Jr.1 1Department of Biology and Neuroscience Program, Lafayette College, 2Department of Information Technology, Computer Science, and Digital Media, Juniata College This protocol describes how to use an in vitro isolated turtle head preparation to measure the kinematics of their eye movements. After removal of the brain from the cranium, cranial nerves can be stimulated with currents to quantify rotations of the eye and changes in pupil sizes. Environment A Simple Approach to Manipulate Dissolved Oxygen for Animal Behavior Observations Christopher J. Grant1, Christopher J. McLimans1 1Department of Biology, Juniata College This article describes a simple and reproducible protocol to manipulate dissolved oxygen conditions in a laboratory setting for animal behavior studies. This protocol may be used in both teaching and research laboratory settings to evaluate organismal response of macroinvertebrates, fishes, or amphibians to changes in dissolved oxygen concentration.