Muhlenberg College 1 article published in JoVE Biochemistry Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory Keri L Colabroy1, Katlyn Mayer1 1Department of Chemistry, Muhlenberg College Here we present a protocol for the benchtop immobilized metal affinity chromatography purification and subsequent reconstitution of a polyhistidine tagged, non-heme iron binding dioxygenase suitable for the undergraduate teaching laboratory.