Indiana University of Pennsylvania 2 articles published in JoVE Developmental Biology In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development Sagar N. Kasar1, Sarah A. Grandinette1, Scott D. Semelsberger1, Cuong Q. Diep1 1Department of Biology, Indiana University of Pennsylvania The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development. Immunology and Infection Zebrafish as a Model to Assess the Teratogenic Potential of Nitrite Vishal Keshari1, Basma Adeeb1, Alison E. Simmons1, Thomas W. Simmons1, Cuong Q. Diep1 1Department of Biology, Indiana University of Pennsylvania Exposure to teratogens may cause birth defects. Zebrafish are useful for determining the teratogenic potential of chemicals. We demonstrate the utility of zebrafish by exposing embryos to various levels of nitrite and also at different times of exposure. We show that nitrite can be toxic and cause severe developmental defects.