Penn State Hershey Cancer Institute 1 article published in JoVE Developmental Biology Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells Aflah Hanafiah1, Zhuangzhuang Geng1, Qiang Wang1, Zhonghua Gao1 1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.
