Fox Chase Cancer Center View Institution's Website 11 articles published in JoVE Cancer Research Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion Madeline Friedman-DeLuca1,2, Prachiben P. Patel1,2, Burcu Karadal-Ferrena1,2, Nicole D. Barth1,2, Camille L. Duran1,2, Xianjun Ye1,2,3, Michael Papanicolaou2,4, John S. Condeelis2,3,4,5,6,7, Maja H. Oktay1,2,3,5,6,7, Lucia Borriello*1,2,8, David Entenberg*1,2,3,5,6 1Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, 2Cancer Dormancy and Tumor Microenvironment Institute, Albert Einstein College of Medicine/Montefiore Medical Center, 3Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, 4Department of Cell Biology, Albert Einstein College of Medicine/Montefiore Medical Center, 5Montefiore Einstein Cancer Center, Albert Einstein College of Medicine/Montefiore Medical Center, 6Integrated Imaging Program for Cancer Research, Albert Einstein College of Medicine/Montefiore Medical Center, 7Department of Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, 8Department of Cancer and Cellular Biology, Lewis Katz School of Medicine, Fox Chase Cancer Center We present a method for studying tumor cell redissemination from lung metastases involving a surgical protocol for the selective photoconversion of lung metastases, followed by the identification of redisseminated tumor cells in tertiary organs. Cancer Research Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids Samuel Flashner*1, Cecilia Martin*1,2, Norihiro Matsuura1, Masataka Shimonosono1, Yasuto Tomita1, Masaki Morimoto1, Ogoegbunam Okolo1, Victoria X. Yu1,3, Anuraag S. Parikh1,3, Andres J. P. Klein-Szanto4, Kelley Yan1,2, Joel T. Gabre1,5, Chao Lu1,6, Fatemeh Momen-Heravi1,7, Anil K. Rustgi1,5, Hiroshi Nakagawa1,2,5 1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University This protocol describes the key steps to generate and characterize murine oral-esophageal 3D organoids that represent normal, preneoplastic, and squamous cell carcinoma lesions induced via chemical carcinogenesis. Biology Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model Louisiane Perrin1, Theodore Tucker1, Bojana Gligorijevic1,2 1Bioengineering Department, Temple University, 2Cancer Biology Program, Fox Chase Cancer Center Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion. Cancer Research Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia Daniela Di Marcantonio1, Stephen M. Sykes1 1Blood Cell Development and Function Program, Fox Chase Cancer Center We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9. Biochemistry Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay Sofiia Karchugina1, Jonathan Chernoff1 1Fox Chase Cancer Center Here, we show how to use a Proximity Ligation Assay (PLA) to visualize MST1/MST2 heterodimerization in fixed cells with high sensitivity. Medicine Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice Joely D. Jacobs1, Elizabeth A. Hopper-Borge1 1Program in Developmental Therapeutics, Fox Chase Cancer Center This method was developed with the goal of delivering a steady drug solution via the carotid artery, to assess the pharmacokinetics of novel drugs in mouse models. Medicine Minimal Erythema Dose (MED) Testing Carolyn J. Heckman1, Rachel Chandler2, Jacqueline D. Kloss3, Amy Benson2, Deborah Rooney2, Teja Munshi1, Susan D. Darlow1, Clifford Perlis4, Sharon L. Manne5, David W. Oslin2 1Cancer Prevention and Control Program, Fox Chase Cancer Center, 2Department of Psychiatry, University of Pennsylvania, 3Department of Psychology, Drexel University, 4Department of Medicine, Fox Chase Cancer Center, 5Cancer Prevention and Control Program, The Cancer Institute of New Jersey This article describes how to conduct minimal erythema dose (MED) testing in order to determine the lowest dose of ultraviolet radiation that will cause erythema (burning) when administered to an individual. Medicine An in vivo Assay to Test Blood Vessel Permeability Maria Radu1, Jonathan Chernoff1 1Fox Chase Cancer Center We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces. Neuroscience Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae Diana Zitserman1, Fabrice Roegiers1 1Epigenetics and Progenitor Cells Keystone, Fox Chase Cancer Center In this video, we describe a method for live cell imaging of asymmetrically dividing sensory organ progenitor cells and epidermal cells in intact Drosophila pupae Immunology and Infection An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses Selene Nunez-Cruz1, Denise C. Connolly2, Nathalie Scholler1 1Penn Ovarian Cancer Research Center, Center for Research on Reproduction and Womans Health, Department of Obstetrics and Gynecology, University of Pennsylvania-School of Medicine, 2Women's Cancer Program, Fox Chase Cancer Center To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging. Biology In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer Alexis B. Cordero1, Youngjoo Kwon1, Xiang Hua2, Andrew K. Godwin1 1Department of Medical Oncology, Women's Cancer Program, 2Transgenic Mouse Facility, Fox Chase Cancer Center Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
