Oak Ridge Institute of Science and Education 2 articles published in JoVE Cancer Research Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors Elizabeth M. Corteselli1, James M. Samet2, Eugene A. Gibbs-Flournoy2,3 1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 2Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education This manuscript describes the use of genetically-encoded fluorogenic reporters in an application of live-cell imaging for the examination of xenobiotic-induced oxidative stress. This experimental approach offers unparalleled spatiotemporal resolution, sensitivity, and specificity while avoiding many of the shortcomings of conventional methods used for the detection of toxicological oxidative stress. Environment Continuous Instream Monitoring of Nutrients and Sediment in Agricultural Watersheds Niroj Aryal1,2, Michele L. Reba2 1Oak Ridge Institute of Science and Education (ORISE), 2Delta Water Management Research Unit, United States Department of Agriculture, Agriculture Research Unit USDA-ARS With the advancement of technology and the rise in end-user expectations, the need and use of higher temporal resolution data for pollutant load estimation has increased. This protocol describes a method for continuous in situ water quality monitoring to obtain higher temporal resolution data for informed water resource management decisions.
