University of Texas Health Science Center 3 articles published in JoVE Developmental Biology Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells Andrew Berical1,2, Mary Lou Beermann2, Shingo Suzuki3, Jake LeSuer2, Taylor Matte2, Brian Davis3, Darrell Kotton1,2, Finn Hawkins1,2 1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center Recent advances in human induced pluripotent stem cell differentiation protocols allow for the stepwise derivation of organ-specific cell types. Here, we provide detailed steps for the maintenance and expansion of iPSC-derived airway basal cells and their differentiation into a mucociliary epithelium in air-liquid interface cultures. Behavior A Community-based Stress Management Program: Using Wearable Devices to Assess Whole Body Physiological Responses in Non-laboratory Settings Robert Carter III1, Kirtigandha Salwe Carter2, John Holliday3, Alice Holliday3, Carlton Keith Harrison4 1Department of Emergency Medicine, The University of Texas Health Science Center, 2Department of Integrative Physiology, The University of North Texas Health Science Center, 3Works of Wonder International, 4DeVos Graduate Sports Business Management Program, University of Central Florida Stress is an unavoidable and persistent component of life and holistic approaches for its management are being considered. A standardized methodology was created to demonstrate the feasibility of a breath-based stress management protocol that can be used with commercially available portable technology. Biochemistry High Precision FRET at Single-molecule Level for Biomolecule Structure Determination Junyan Ma1, Inna S. Yanez-Orozco2, Soheila Rezaei Adariani2, Drew Dolino3, Vasanthi Jayaraman3, Hugo Sanabria2 1Department of Chemistry, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences, University of Texas Health Science Center A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.
