Resonant Sensors Incorporated 1 article published in JoVE Immunology and Infection Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology Jon A. Weidanz1, Krysten L. Doll1, Soumya Mohana-Sundaram1, Timea Wichner1, Devin B. Lowe1, Susanne Gimlin2, Debra Wawro Weidanz2, Robert Magnusson2,3, Oriana E. Hawkins1 1Experimmune, A Center for Immunotherapeutic Development, Department of Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences Center, 2Resonant Sensors Incorporated, 3University of Texas Arlington Intact class I HLA/peptide complexes are shed by cancer cells, representing a potential relevant cancer biomarker. Utilizing label-free sensor technology and T-cell receptor mimicking monoclonal antibodies, detection of shed MIF/HLA-A*02:01 complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma is demonstrated, enabling development of a novel cancer diagnostic platform.
